Obtusifoliol is primarily involved in the biosynthesis of sterols in plants and fungi, playing a key role in maintaining cell membrane integrity and fluidity. It serves as an intermediate in the production of essential phytosterols, which are crucial for plant growth and development. Due to its position in the sterol pathway, obtusifoliol is also a target for certain fungicides, particularly in agriculture, where inhibitors of its conversion are used to disrupt ergosterol synthesis in pathogenic fungi, thereby controlling fungal infections in crops. Its presence and metabolic pathway are studied to develop more effective and selective antifungal agents with minimal impact on non-target organisms.