G418 Sulfate (700U/mg) is widely used in molecular biology and genetic research as a selective agent. It is commonly applied in cell culture experiments to select and maintain eukaryotic cells that have been genetically modified to express the neomycin resistance gene. When added to the culture medium, it inhibits protein synthesis in non-resistant cells, effectively killing them, while allowing the growth of resistant cells. This makes it a valuable tool for stable transfection studies, where researchers aim to integrate foreign DNA into the host genome. Additionally, it is used in the development of stable cell lines for producing recombinant proteins or studying gene function. Its effectiveness in selecting genetically engineered cells has made it a staple in biotechnology and pharmaceutical research.