Used primarily as a selective agent in cell culture and molecular biology to isolate genetically modified cells. Cells transfected with a resistance gene to Hygromycin B survive exposure, while non-resistant cells die. Commonly used in both mammalian and bacterial systems for stable cell line development. Also applied in veterinary medicine as an antiparasitic agent, particularly effective against nematodes and bacteria in livestock. Its ability to inhibit protein synthesis makes it valuable in research settings for controlling contamination and ensuring genetic selection accuracy.