Hygromycin B solution is widely used as a selective agent in cell culture and molecular biology, particularly for selecting genetically engineered cells. It is effective in both prokaryotic and eukaryotic systems, commonly used to select transformed bacteria, yeast, plant, and mammalian cells that carry resistance genes, such as the hph gene. Its mechanism involves inhibiting protein synthesis by binding to the 30S subunit of the bacterial ribosome, making it ideal for maintaining selection pressure in cultures over time. Due to its broad-spectrum activity and stability in culture media, it is a preferred antibiotic in stable transfection experiments and long-term cell line development. It is also used in veterinary medicine as an antiparasitic agent, particularly against nematodes and bacteria in livestock.