Hygromycin B solution is widely used as a selective agent in cell culture and molecular biology, particularly for selecting genetically engineered mammalian, plant, and bacterial cells. It works by inhibiting protein synthesis in susceptible cells, allowing only those expressing a resistance gene (such as hph or aph) to survive. This makes it valuable in stable transfection experiments where researchers need to isolate cells that have successfully incorporated a desired gene construct. Due to its effectiveness and relatively low toxicity to resistant cells, it is commonly used in generating transgenic cell lines and in gene expression studies. It is also employed in veterinary medicine as an antiparasitic agent, effective against certain nematodes and bacteria in livestock.