Hygromycin B solution is widely used as a selective agent in cell culture and molecular biology, particularly for the selection of eukaryotic cells (such as mammalian, yeast, or plant cells) that have been genetically engineered with a resistance gene. It inhibits protein synthesis in a broad range of organisms, including bacteria, fungi, and higher eukaryotes, making it effective for eliminating non-resistant cells during stable transfection experiments. In research settings, it is commonly added to growth media to maintain selective pressure, ensuring only cells expressing the hygromycin resistance gene (like hph or aph) survive. This makes it essential in gene knockout studies, transgenic cell line development, and recombinant protein production. Its ability to act on both dividing and non-dividing cells enhances its utility in various experimental systems. Due to its stability in solution, Hygromycin B is convenient for long-term selection processes.