Hygromycin B is widely used as a selective agent in cell culture and molecular biology, particularly in the selection of eukaryotic cells transfected with resistance genes. It is commonly applied in the generation of stable cell lines, where cells expressing the hygromycin resistance gene (such as hph or aph) survive in the presence of the antibiotic while non-resistant cells die. This makes it essential in gene expression studies, gene knockout experiments, and recombinant protein production. In microbiology and biotechnology, Hygromycin B is used to maintain plasmid constructs in mammalian, yeast, and other eukaryotic systems. Its ability to inhibit protein synthesis by interfering with ribosomal function ensures effective selection pressure. The standard solution format allows for precise dosing and consistent results in screening protocols, making it valuable in both research and industrial applications.