Hygromycin B standard solution is widely used in molecular biology and genetic engineering as a reference standard for quantification and calibration. It is essential in the development and validation of analytical methods, particularly in HPLC and mass spectrometry, to ensure accurate measurement of hygromycin B in various samples. This standard solution is also critical in quality control processes during the production of antibiotics and in research involving selection of genetically modified cells, where hygromycin resistance genes are used as markers. Its precise concentration allows for reliable and reproducible results in assay validation, ensuring consistency in both research and industrial applications.