BISINDOLYMALEIMIDE IV is primarily used in biochemical research as a potent and selective inhibitor of protein kinase C (PKC). It is widely applied in studies to understand the role of PKC in cellular signaling pathways, particularly in processes like cell proliferation, differentiation, and apoptosis. Researchers utilize this compound to investigate the mechanisms of PKC-mediated responses in various cell types, including cancer cells, to explore potential therapeutic targets. Additionally, it serves as a valuable tool in pharmacological studies to evaluate the effects of PKC inhibition on disease models, such as cardiovascular disorders, neurological conditions, and inflammatory diseases. Its specificity makes it a crucial reagent in elucidating the complex roles of PKC isoforms in health and disease.