KT5720 is primarily used in research settings to study cellular processes and signaling pathways. It acts as a potent and selective inhibitor of protein kinase A (PKA), an enzyme involved in various physiological functions. By inhibiting PKA, KT5720 helps researchers understand the role of this kinase in cellular responses, such as metabolism, gene expression, and cell growth. It is particularly valuable in studies related to cardiac function, neuronal signaling, and cancer research, where PKA activity is often implicated. Additionally, KT5720 is used to explore the interplay between PKA and other signaling pathways, providing insights into complex biological mechanisms. Its application is limited to laboratory experiments and is not used in clinical or therapeutic contexts.