HA-100 hydrochloride is primarily utilized in research settings as a potent and selective inhibitor of protein kinases, particularly targeting cAMP-dependent protein kinase (PKA) and protein kinase C (PKC). Its application is significant in studying cellular signaling pathways, as it helps elucidate the roles of these kinases in various biological processes. Researchers use it to investigate mechanisms related to cell proliferation, differentiation, and apoptosis, providing insights into diseases such as cancer and neurological disorders. Additionally, it serves as a valuable tool in pharmacological studies to develop targeted therapies by understanding kinase inhibition effects. Its use is confined to laboratory environments, where it aids in advancing knowledge in biochemistry and molecular biology.