This compound is primarily utilized in pharmaceutical research and development as an intermediate in the synthesis of more complex molecules. Its structure, featuring a nitropyridine and piperazine moiety, makes it valuable for designing drugs targeting neurological disorders, particularly those involving the central nervous system. It is often employed in the creation of potential inhibitors for enzymes or receptors associated with conditions like depression, anxiety, or neurodegenerative diseases. Additionally, its nitro group allows for further chemical modifications, enabling researchers to explore diverse pharmacological activities. The tert-butyl carboxylate group provides stability and aids in the purification process during synthesis. Its applications are largely confined to laboratory settings, where it contributes to the discovery of novel therapeutic agents.