Used as a key intermediate in the synthesis of pharmaceuticals, particularly in the development of peptide-based drugs and cyclic dipeptide derivatives. Its rigid piperazine dione backbone supports structural stability in drug design, enhancing metabolic resistance and bioavailability. Also explored in the preparation of bioactive compounds with antimicrobial and antitumor properties. Additionally, serves as a building block in organic synthesis for creating constrained heterocyclic systems used in medicinal chemistry research.