Di-tert-butyl piperazine-1,4-dicarboxylate is primarily used as an intermediate in organic synthesis, particularly in the pharmaceutical industry. It plays a crucial role in the development of various active pharmaceutical ingredients (APIs) and drug candidates. Its structure allows it to act as a building block for the synthesis of complex molecules, especially those requiring piperazine derivatives. Additionally, it is employed in the preparation of compounds with potential therapeutic applications, such as anti-cancer agents, antimicrobials, and central nervous system (CNS) drugs. Its tert-butyl groups provide steric protection, making it valuable in multi-step synthetic processes where selective reactivity is needed.