CZC-25146 hydrochloride is primarily used in biochemical research as a potent and selective inhibitor of the protein kinase PIM1. This compound is valuable in studying the role of PIM1 in cellular processes such as cell proliferation, survival, and differentiation. Researchers utilize it to investigate the potential therapeutic effects of PIM1 inhibition in various cancers, as PIM1 is often overexpressed in malignancies like leukemia, prostate cancer, and solid tumors. Its application helps in understanding signaling pathways and developing targeted cancer therapies. Additionally, it serves as a tool compound in preclinical studies to evaluate the efficacy and mechanisms of PIM1 inhibitors in disease models.