CX-6258 HCl is primarily utilized in research settings as a potent and selective inhibitor of Pim kinases, which are involved in various cellular processes including cell proliferation, survival, and differentiation. Its application is significant in studying the role of Pim kinases in cancer biology, as these kinases are often overexpressed in several types of cancers, including leukemia, prostate cancer, and solid tumors. Researchers use CX-6258 HCl to investigate the potential therapeutic effects of Pim kinase inhibition, particularly in reducing tumor growth and enhancing the efficacy of other cancer treatments. Additionally, it is employed in preclinical studies to explore its impact on signaling pathways and to identify potential biomarkers for cancer therapy.