CX-6258 hydrochloride hydrate is primarily utilized in research settings as a potent and selective inhibitor of Pim kinases, which are involved in cell survival, proliferation, and differentiation. Its application is significant in studying cancer biology, as Pim kinases are often overexpressed in various malignancies, including leukemia, prostate cancer, and solid tumors. Researchers use this compound to explore its effects on tumor growth inhibition and to investigate potential therapeutic strategies targeting Pim kinase pathways. Additionally, it serves as a valuable tool in understanding the role of Pim kinases in immune regulation and hematopoiesis, contributing to the development of novel treatments for hematologic disorders and immune-related diseases.