PI-828 is primarily utilized in research settings as a potent inhibitor of phosphoinositide 3-kinase (PI3K), a key enzyme involved in cellular signaling pathways. Its application is significant in studying cancer biology, as PI3K signaling is often dysregulated in various types of tumors. Researchers use PI-828 to block this pathway, helping to understand its role in cell growth, survival, and metabolism. This compound is also valuable in exploring potential therapeutic strategies targeting PI3K in cancer treatment. Additionally, PI-828 is employed in biochemical assays to investigate the mechanisms of PI3K-related diseases and to screen for novel inhibitors with improved efficacy and selectivity.