Vps34-IN-1 is primarily used in research settings to study the role of Vps34, a class III phosphoinositide 3-kinase, in cellular processes. It is a potent and selective inhibitor of Vps34, making it a valuable tool for understanding autophagy, vesicle trafficking, and endosomal sorting. Researchers utilize this compound to investigate how inhibition of Vps34 affects cellular signaling pathways, nutrient sensing, and cancer cell survival. Its application extends to exploring potential therapeutic strategies for diseases where Vps34 activity is implicated, such as cancer, neurodegenerative disorders, and immune-related conditions. By blocking Vps34, it helps elucidate the enzyme's contribution to cellular homeostasis and disease progression.