Atractylenolide III is widely utilized in traditional Chinese medicine for its anti-inflammatory and immunomodulatory properties. It is commonly applied in the treatment of gastrointestinal disorders, such as irritable bowel syndrome and ulcerative colitis, due to its ability to reduce inflammation and promote gut health. Additionally, it is used in managing autoimmune diseases by regulating immune responses and suppressing excessive immune activity. In cancer research, Atractylenolide III has shown potential as an adjunct therapy, as it inhibits the proliferation of certain cancer cells and enhances the efficacy of chemotherapy drugs. Its antioxidant effects also make it beneficial in protecting cells from oxidative stress, contributing to its use in anti-aging and skincare formulations.