Cyclopamine is primarily used in research to study the Hedgehog signaling pathway, which plays a critical role in cell growth and differentiation. By inhibiting this pathway, it helps scientists understand its role in various biological processes, including embryonic development and cancer progression. In cancer research, cyclopamine is particularly valuable for investigating its potential to suppress the growth of tumors that are dependent on Hedgehog signaling, such as basal cell carcinoma and medulloblastoma. Additionally, it serves as a tool in developmental biology to study the effects of disrupted Hedgehog signaling on tissue and organ formation. Its application extends to drug discovery, where it aids in identifying and developing new therapeutic agents targeting this pathway.