Timosaponin A-III is widely studied for its potential anti-cancer properties, particularly in inhibiting the growth of various tumor cells such as breast, colon, and liver cancer. It induces apoptosis and autophagy in cancer cells through modulation of key signaling pathways like PI3K/AKT and MAPK. It also shows neuroprotective effects, making it a candidate for research in neurodegenerative diseases such as Alzheimer’s, where it may help reduce amyloid-beta accumulation and improve cognitive function. Additionally, Timosaponin A-III exhibits anti-inflammatory and antioxidant activities, contributing to its use in studies related to metabolic disorders and aging. Its ability to enhance insulin sensitivity suggests potential applications in managing type 2 diabetes. Research is ongoing to explore its bioavailability and therapeutic efficacy in preclinical models.