Escholine chloride is primarily used in research settings as a neuromuscular blocking agent. It functions by interfering with nerve-muscle communication, making it useful in studies related to acetylcholine receptors and synaptic transmission. Due to its paralytic effects, it has been employed in physiological experiments to induce controlled muscle relaxation, particularly in isolated tissue preparations. Its application is limited to laboratory and experimental pharmacology, as it is not used clinically in humans or animals for therapeutic purposes. The compound also serves as a reference standard in toxicology studies involving cholinergic systems.