Dioscin is widely studied for its potential therapeutic applications, particularly in cancer research. It exhibits strong anti-tumor activity by inducing apoptosis and inhibiting proliferation in various cancer cell lines, including liver, lung, and breast cancer. Its ability to modulate key signaling pathways such as PI3K/AKT and MAPK makes it a promising candidate for anticancer drug development. In addition to oncology, dioscin shows significant anti-inflammatory and antioxidant effects, which are beneficial in managing chronic inflammatory diseases and oxidative stress-related conditions. It has also demonstrated hepatoprotective properties, helping protect the liver from damage caused by toxins and reducing fibrosis. Another important application is in cardiovascular health, where dioscin helps lower cholesterol levels by interfering with intestinal absorption of lipids. This effect supports its potential use in treating hyperlipidemia and preventing atherosclerosis. Due to its diverse pharmacological activities, dioscin is also used as a reference compound in quality control and standardization of herbal medicines, especially those derived from Dioscorea species.