Used primarily in neuroscience research to block GABA_A receptors, enabling the study of inhibitory neurotransmission. It induces neuronal excitation and can trigger seizures in experimental models, making it valuable for investigating epilepsy mechanisms. Also employed to examine the role of GABAergic signaling in synaptic plasticity, anxiety, and muscle tone regulation. Due to its potent antagonistic effects, it is used in controlled settings to map neural circuits and receptor function.