Escholine chloride is primarily used in research and laboratory settings as a pharmacological tool to study the effects of acetylcholine receptors. It acts as a selective agonist, helping researchers understand the signaling pathways and physiological responses mediated by these receptors. This compound is particularly valuable in neuroscience and pharmacology for investigating the role of acetylcholine in the nervous system, including its impact on muscle contraction, memory, and cognitive functions. Additionally, it is utilized in experiments to explore potential therapeutic applications for conditions related to acetylcholine receptor dysfunction. Its specificity makes it a useful compound for targeted studies in cellular and molecular biology.