This compound is primarily utilized in photoaffinity labeling studies, a technique used to investigate molecular interactions in biological systems. Its diazirine group, when exposed to ultraviolet light, generates a highly reactive carbene intermediate that can form covalent bonds with nearby molecules. This property makes it valuable for probing protein-ligand interactions, mapping binding sites, and studying the structure and function of biomolecules. The iodoethyl group enhances its utility by allowing further functionalization or conjugation to specific targets, while the alkyne moiety enables click chemistry for downstream detection or purification. Its application is particularly significant in drug discovery and biochemical research, where understanding molecular interactions at a detailed level is crucial.