This compound is primarily utilized in photoaffinity labeling, a technique employed in biochemical research to study protein-ligand interactions. Its diazirine group, when exposed to UV light, generates a highly reactive carbene species that can form covalent bonds with nearby molecules, effectively "capturing" transient interactions. This makes it valuable for identifying binding sites on proteins or understanding molecular mechanisms in biological systems. Additionally, its trifluoromethyl group enhances stability and reactivity, making it suitable for probing complex biological pathways. Researchers often use it to map protein-protein interactions, enzyme active sites, or drug-target binding, providing insights into cellular processes and aiding drug discovery efforts.