This chemical is primarily utilized in photoaffinity labeling studies to investigate molecular interactions and binding sites. Its diazirine group, when exposed to UV light, generates a highly reactive carbene intermediate that can form covalent bonds with nearby molecules, enabling the identification and mapping of protein-ligand interactions. It is particularly valuable in proteomics and drug discovery for studying protein structures, receptor binding, and enzyme active sites. Additionally, it aids in the development of targeted therapies by providing insights into the molecular mechanisms of drug action. Its trifluoromethyl group enhances stability and reactivity, making it a versatile tool in biochemical research.