Used primarily as a photoactivatable crosslinking agent in biochemical research. Its main application lies in photoaffinity labeling, where it helps identify and study molecular interactions between proteins, nucleic acids, and small molecule ligands. Upon exposure to UV light, the diazirine group generates highly reactive carbenes that form covalent bonds with nearby molecules, capturing transient or weak interactions that are difficult to study by other methods. This makes it valuable in mapping binding sites, studying protein-protein interactions, and identifying unknown targets of bioactive compounds. The primary amine group allows for easy conjugation to carboxylated or activated ester-containing molecules, enabling its incorporation into probes, drugs, or tags for downstream detection. Its stability in dark conditions and selective activation by light provide precise temporal control, making it ideal for use in complex biological systems.