Used primarily as a photoaffinity labeling agent in biochemical and pharmaceutical research. Upon exposure to UV light, it generates a highly reactive nitrene species that inserts covalently into nearby C–H or X–H bonds, allowing researchers to capture and study transient interactions between biomolecules such as proteins, enzymes, and receptors. Its fluorinated and nitro-substituted aromatic structure enhances both reactivity and detection sensitivity, making it valuable in probe design for target identification, mapping binding sites, and studying protein-ligand interactions. Also employed in surface modification and materials science for functionalizing polymers or nanomaterials through covalent attachment.