Used primarily as a photoaffinity labeling agent in biochemical and pharmacological research. Its diazirine group generates highly reactive carbene species upon exposure to UV light, enabling covalent binding to nearby biomolecules such as proteins or nucleic acids. This property makes it valuable for mapping molecular interactions, identifying drug targets, and studying receptor-ligand binding sites. The trifluoromethyl group enhances stability and improves lipophilicity, aiding in membrane permeability for cellular studies. The hydroxymethyl group facilitates easy modification and conjugation with other molecules. Commonly incorporated into probe molecules to trace biological pathways and validate binding specificity in complex biological systems.