Used primarily as a photoaffinity labeling agent in biochemical and pharmacological research. Its diazirine ring generates highly reactive carbenes upon exposure to UV light, enabling covalent binding to nearby biomolecules. This property makes it valuable for mapping drug-protein interactions, identifying binding sites, and studying receptor-ligand relationships. The bromo and alkyne functional groups allow for further derivatization or conjugation via click chemistry or nucleophilic substitution, facilitating incorporation into complex molecular probes. Commonly applied in proteomics and target identification studies where precise molecular crosslinking is required.