Used primarily as a photoactivatable crosslinking agent in biochemical and molecular biology research. Its main application lies in photoaffinity labeling, where it enables the identification of molecular interactions between proteins, nucleic acids, and small molecule ligands. Upon exposure to UV light, the diazirine group generates highly reactive carbenes that form covalent bonds with nearby molecules, capturing transient or weak interactions. The alkyne handle allows for downstream detection or purification via click chemistry, such as copper-catalyzed azide-alkyne cycloaddition (CuAAC), enabling selective conjugation with fluorescent tags or biotin. This dual functionality makes it valuable for proteomics studies, target identification, and mapping binding sites in complex biological systems.