Used primarily as a photoactivatable crosslinking agent in biochemical research. The diazirine ring generates highly reactive carbenes upon exposure to UV light, enabling covalent bonding with nearby molecules. This property makes it valuable for studying protein-protein interactions, mapping binding sites, and identifying molecular targets in complex biological systems. Its hydroxyl group allows for further chemical modification or conjugation, enhancing utility in probe design. Commonly employed in affinity labeling and photoaffinity labeling experiments to capture transient or weak interactions in live cells or purified systems.