Used primarily as a photoaffinity labeling agent in biochemical and pharmacological research. Its diazirine group generates highly reactive carbenes upon UV light exposure, enabling covalent binding to nearby biomolecules such as proteins or nucleic acids. The ethynyl group allows for downstream conjugation via click chemistry, facilitating detection, isolation, or enrichment of labeled targets. This compound is especially valuable in studying protein-ligand interactions, mapping binding sites, and identifying unknown receptors in complex biological systems.