Used as a photoaffinity labeling agent in biochemical research, enabling the study of protein-ligand interactions. Upon UV irradiation, the azido group generates a nitrene radical that forms covalent bonds with nearby biomolecules, helping identify drug binding sites or map interaction domains in complex biological systems. Its triazolidinedione core can participate in hydrogen bonding, enhancing target specificity. Commonly applied in probe development for proteomics and receptor studies.