Used extensively in photoaffinity labeling studies to investigate protein-ligand interactions. Its diazirine group, when exposed to UV light, forms a highly reactive carbene that can insert into nearby molecules, enabling the study of binding sites and molecular interactions. Commonly applied in biochemical research to map protein structures and identify binding partners. Also utilized in the development of probes for studying enzyme mechanisms and receptor functions. Its trifluoromethyl group enhances stability and reactivity, making it a valuable tool in photochemical and bioconjugation applications.