This chemical is widely used in photoaffinity labeling studies, a technique employed to investigate molecular interactions within biological systems. Its diazirine group, when exposed to ultraviolet light, generates a highly reactive carbene intermediate that can form covalent bonds with nearby molecules. This property makes it invaluable for mapping protein-ligand interactions, identifying binding sites, and studying protein-protein interactions. Additionally, it is utilized in the development of probes for studying enzyme mechanisms and receptor-ligand dynamics. Its trifluoromethyl group enhances its stability and reactivity, making it a preferred choice in biochemical research and drug discovery processes.