Used in research to study cellular signaling pathways, particularly those involving PI(3,5)P₂, a phosphoinositide lipid that plays key roles in endolysosomal trafficking, autophagy, ion channel regulation, and membrane dynamics. This compound serves as a synthetic analog of PI(3,5)P₂, an important lipid second messenger in intracellular membranes. It is utilized in in vitro assays to examine the activity and regulation of PI(3,5)P₂-binding proteins, such as the kinase PIKfyve, the phosphatase FIG4, and ion channels like TRPML1. The defined dioleoyl acyl chain composition ensures reproducible results in lipid-protein interaction studies and liposome-based models of endosomal/lysosomal membranes. Commonly applied in investigations of vacuolar/lysosomal function, nutrient sensing, insulin signaling, and diseases including cancer, neurodegeneration, and lysosomal storage disorders.