Bromo enol lactone is primarily used in biochemical research as a potent and selective inhibitor of calcium-independent phospholipase A2 (iPLA2). This enzyme plays a significant role in various cellular processes, including membrane remodeling, signal transduction, and the production of lipid mediators. By inhibiting iPLA2, bromo enol lactone helps researchers study the enzyme's function and its impact on cellular mechanisms, particularly in the context of inflammation, neurodegenerative diseases, and cardiovascular conditions. Its application is crucial in understanding the pathways involved in these diseases and in developing potential therapeutic strategies. Additionally, it is utilized in studies focusing on lipid metabolism and oxidative stress, providing insights into the role of iPLA2 in these processes.