Used in cell signaling research to study phosphoinositide pathways, particularly those involving phosphatidylinositol 3-phosphate (PI(3)P) in processes such as endocytosis, autophagy, and intracellular membrane trafficking. Serves as a synthetic short-chain analog of PI(3)P, facilitating the recruitment and activation of downstream effectors containing FYVE, PX, or other PI(3)P-binding domains in vitro. Employed in lipid kinase and phosphatase assays to evaluate enzyme activity and inhibitor potency specific to PI(3)P metabolism. Also utilized in membrane trafficking and lipid-protein interaction studies due to its ability to bind and recruit phosphoinositide-specific domains to lipid membranes in experimental systems.