Used in cell signaling research to study phosphoinositide pathways involved in membrane dynamics and intracellular communication. Serves as a soluble analog of PIP2 (phosphatidylinositol 4,5-bisphosphate), allowing controlled activation of downstream effectors such as ion channels, phospholipase C, and cytoskeletal regulatory proteins. Commonly applied in in vitro assays to investigate G-protein coupled receptor signaling, vesicle trafficking, and calcium release mechanisms. Its diacylglycerol chain is shortened for improved water solubility, making it suitable for experiments requiring rapid delivery and consistent dispersion in aqueous buffers.