Used in lipid signaling research to study phosphoinositide-dependent cellular processes. Serves as a synthetic analog of PI(3,4)P2, a key lipid second messenger involved in regulating membrane trafficking, cell survival, and insulin signaling. Employed in in vitro assays to investigate the activity and specificity of phosphoinositide-binding domains, such as PH, PX, and FYVE domains. Also utilized in liposome formulations to mimic physiological membranes and study protein-lipid interactions. Its defined acyl chain composition (di-oleoyl) ensures consistent biophysical properties in membrane studies.