Used in cell signaling research to study phosphoinositide pathways, particularly those involving PI(3,4)P2, which regulates cellular processes such as membrane trafficking, cytoskeletal reorganization, and cell survival. Serves as a soluble analog of endogenous phosphoinositides in lipid-protein interaction studies, enabling investigation of PH and PX domain-containing proteins. Applied in in vitro assays to activate or inhibit specific kinases and phosphatases involved in signal transduction. Its diacyl structure enhances membrane permeability and stability in experimental systems, making it suitable for biochemical and biophysical analyses.