β-Glycerol phosphate disodium salt is widely used in biochemical and cell culture applications. It serves as a substrate for studying enzyme kinetics, particularly for phosphatases and kinases, due to its role in phosphate transfer reactions. In cell culture, it is utilized as a source of inorganic phosphate, which is essential for cellular metabolism and energy production. Additionally, it is employed in research related to bone and cartilage formation, as it can influence osteoblast differentiation and mineralization processes. Its buffering capacity also makes it suitable for maintaining pH stability in various experimental setups.