Used as a substrate in enzymatic assays, particularly for alkaline phosphatase, to detect enzyme activity in biological samples. It is applied in biochemical research to study enzyme kinetics and mechanisms. Also employed in diagnostic kits for measuring alkaline phosphatase levels in clinical settings, aiding in the detection of liver and bone disorders. In histochemistry, it helps visualize enzyme localization in tissue samples. Additionally, it serves as a reagent in molecular biology techniques for labeling and detection purposes.