Calyculin A is widely used in biochemical research as a potent inhibitor of protein phosphatases, particularly PP1 and PP2A. Its primary application is in studying cellular signaling pathways, as it induces hyperphosphorylation of proteins, allowing researchers to investigate the role of phosphorylation in processes like cell cycle regulation, apoptosis, and cytoskeleton dynamics. It is also utilized to mimic stress conditions in cells, providing insights into stress response mechanisms. Additionally, Calyculin A is employed in cancer research to explore its effects on tumor suppression and cell proliferation pathways. Its ability to enhance actin polymerization makes it valuable in studies focused on cell morphology and motility.