CDC25B-IN-2 is primarily used in biochemical research to study the inhibition of the CDC25B phosphatase enzyme, which plays a critical role in cell cycle regulation. By inhibiting this enzyme, researchers can explore its effects on cell proliferation and its potential as a target in cancer therapy. This compound is particularly valuable in understanding how disrupting the cell cycle can lead to the suppression of tumor growth. It is often employed in laboratory settings to investigate the mechanisms of action and efficacy of CDC25B inhibitors in various cancer cell lines. Additionally, it aids in the development of novel therapeutic strategies aimed at controlling uncontrolled cell division in malignancies.