Tautomycetin is primarily utilized in biochemical research due to its potent inhibitory effects on protein phosphatases. It specifically targets type 1 and type 2A protein phosphatases, making it a valuable tool in studying cellular signaling pathways. Researchers employ tautomycetin to investigate the role of these phosphatases in processes like cell cycle regulation, apoptosis, and signal transduction. Its ability to selectively inhibit these enzymes helps in understanding their function in various diseases, including cancer and neurodegenerative disorders. Additionally, tautomycetin is used in studies exploring immune responses, as it can modulate T-cell activation and function. Its application extends to the development of therapeutic strategies targeting phosphatase-related pathways.